Abcam, ab259063, Human PRKD3 (PKC nu/PRKD3) knockout HeLa cell lysate

Size: 1Kit
PRKD3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PRKD3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein kinase C nu also known as PRKD3 is an important serine/threonine kinase located in the protein kinase D family. This protein has a molecular mass of approximately 102 kDa. PRKD3 is mainly expressed in human tissues like the brain heart and lungs. It plays a role in cellular processes by phosphorylating various substrates. The protein can localize to different cellular compartments allowing it to conduct multiple signaling roles effectively.
Biological function summary
PRKD3 influences cellular responses to environmental stress and regulates gene expression. It can promote cell proliferation and survival by being part of a complex signaling network. This protein modulates transcription factors and cellular mechanisms to maintain homeostasis under changing conditions. PRKD3 has interactions with other kinases within its family helping to fine-tune signal transduction processes.
Pathways
PKC nu/PRKD3 integrates into the MAPK signaling cascades essential for proliferative and stress-responses. It also interacts within the NF-kB pathway which regulates immune responses and inflammation. These pathways involve various other proteins like ERKs and IκB kinase positioning PRKD3 as a regulatory node in key cellular activities. This interconnection emphasizes the protein's versatile role in cellular adaptation and signaling.
PRKD3 shows associations with cancer and cardiovascular diseases. The protein's influence over cell growth and apoptosis links it to oncogenic processes making it relevant in cancer research. Additionally its role in heart tissue suggests connections with cardiovascular pathologies. PRKD3 associates with proteins like Bcl-2 in cancer and influences pathways related to heart functions highlighting its relevance in these disorders.