Abcam, ab259098, Human SCNN1D knockout HeLa cell lysate

Size: 1Kit
SCNN1D KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon8 and 2 bp deletion in exon8.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon8 and 2 bp deletion in exon8.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SCNN1D, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SCNN1D gene encodes the delta subunit of the epithelial sodium channel often referred to as ENaC-delta. This subunit forms a part of the multisubunit complex that regulates sodium balance at the epithelial surface. It resides on the cell membrane and plays a mechanical role in facilitating sodium ion transport across epithelial cells. SCNN1D is about 74 kDa in size and is expressed predominantly in renal epithelium and respiratory tissues contributing to sodium homeostasis and fluid balance.
Biological function summary
SCNN1D plays an important role in sodium reabsorption as part of the epithelial sodium channel complex which includes alpha beta and gamma subunits. Together these subunits form a channel responsible for the movement of sodium ions into the cells critical for maintaining electrolyte balance and blood pressure. Its specific contribution enhances the channel's sensitivity and responsiveness to hormonal regulation particularly by aldosterone which modulates electrolyte transport further influencing fluid accumulations in tissues.
Pathways
SCNN1D acts within the aldosterone-regulated sodium reabsorption pathway and the renin-angiotensin-aldosterone system (RAAS). These pathways are essential in regulating blood pressure and fluid balance. SCNN1D works closely with other proteins like the mineralocorticoid receptor which influences ENaC activity by regulating its transcription under the stimulus of aldosterone ensuring facilitating rapid sodium uptake in epithelial cells.
SCNN1D involvement becomes significant in hypertension and cystic fibrosis. Mutations or dysregulation of SCNN1D or its associated pathways can lead to altered blood pressure control due to improper sodium retention. Moreover its role in sodium reabsorption associates this subunit with cystic fibrosis where the balance of ions and fluids in the lungs impacts disease severity. The protein is also linked with cystic fibrosis transmembrane conductance regulator (CFTR) which co-regulates ion transport and channel activity across epithelial surfaces.