Abcam, ab259112, Human SHC4 (SHCD) knockout HeLa cell lysate

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SHC4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 4 bp deletion in exon4.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 4 bp deletion in exon4.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SHC4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SHC4 also known as SHCD is a member of the SHC (Src Homology 2 domain-containing) family and plays an important role in intracellular signaling. The protein has a molecular mass of approximately 72 kDa. SHC4 mainly localizes in the cytoplasm and can translocate to the membrane where it participates in signaling activities. Its expression occurs widely across different tissues but it shows a particularly strong expression in the nervous system indicating a potential role in neural processes.
Biological function summary
SHC4 serves as an adaptor protein that connects activated receptors to downstream signaling pathways. It can associate with various signaling complexes helping to mediate responses to extracellular stimuli. SHC4 transmits signals from receptors like receptor tyrosine kinases (RTKs) and plays a part in cellular functions such as proliferation and differentiation. These attributes highlight its involvement in complex networks that control cellular responses.
Pathways
SHC4 participates in signaling cascades like the MAPK/ERK and PI3K/AKT pathways. It interacts with proteins such as GRB2 which transmits signals from activated RTKs to the Ras protein ultimately influencing cell fate decisions. These pathways are critical for regulating cell cycle progression cellular growth and metabolism demonstrating SHC4's integral function in these processes.
SHC4 associates with conditions impacting the nervous system including neurodevelopmental disorders. Its altered signaling capability may relate to aberrant neural development affecting processes stabilized by SHC4. Moreover SHC4 has been studied in cancer where dysregulation of associated pathways like MAPK/ERK plays a significant role. Its interaction with proteins such as RAS which can act as oncogenes when mutated marks SHC4 as an important protein in oncology research.