Abcam, ab259114, Human SIVA1 (SIVA) knockout HEK-293T cell lysate

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SIVA1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SIVA1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIVA also known as CD27-binding protein SIVA is a pro-apoptotic protein involved in cell death mechanisms. This protein typically weighs around 15 kDa and predominantly locates in the cytoplasm and the nucleus of cells. It expresses in a variety of tissues including immune system organs like the thymus and spleen reflecting its integral role in immune function and development.
Biological function summary
SIVA impacts apoptosis regulation through its interaction with signaling pathways that mediate cell death. It binds to the CD27 receptor a member of the tumor necrosis factor receptor (TNFR) family which is critical for the induction of apoptosis. SIVA is known to interact with other proteins such as those in the Bcl-2 family which further emphasize its role in orchestrating cell death programs.
Pathways
Various apoptotic cascades involve SIVA notably the extrinsic apoptotic signaling pathway linked to the TNFR family. This pathway engages the FasL/Fas and TNFR1 complexes where SIVA functions alongside other apoptosis regulators like caspases. SIVA also interfaces with proteins like TRAFs (tumor necrosis factor receptor-associated factors) which modulate signaling pathways related to immune responses and cell survival.
Research links SIVA to cancer and autoimmune conditions. In cancer SIVA’s modulation of apoptosis can influence tumor progression and chemotherapy resistance often through its interaction with Bcl-2 proteins that inhibit cell death. Similarly in autoimmune diseases where immune cell regulation is disrupted SIVA's role affects T-cell survival and overall immune responses connecting it with disorders where TNFR family signaling plays a part.