Abcam, ab259202, Human TRANK1 knockout A549 cell lysate

Size: 1Kit
TRANK1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon11 and 7 bp deletion in exon11.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon11 and 7 bp deletion in exon11.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TRANK1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRANK1 also known as Tetratricopeptide Repeat And Ankyrin Repeat Containing 1 is a protein with a mass of approximately 190 kDa. This protein features tetratricopeptide repeats which assist in protein-protein interactions. TRANK1 is expressed widely but higher levels appear in brain tissues. It plays roles in the regulation of several cellular processes through its binding capabilities acting as a modular unit that mediates various cellular interactions.
Biological function summary
Several cellular activities rely on TRANK1 due to its ability to participate in multiprotein complexes. It regulates transcription and cell cycle processes impacting how cells grow and respond to environmental signals. By modulating the activity of other proteins TRANK1 enables cells to maintain homeostasis and adapt to changes. These actions suggest a role in normal brain functions where regulatory precision is needed.
Pathways
The TRANK1 protein interacts within the Wnt signaling and stress response pathways. These pathways involve proteins that regulate gene expression in response to extracellular signals affecting cell fate and proliferation. In the Wnt signaling pathway TRANK1 can influence components like β-catenin impacting processes like cell differentiation. It ensures proper signaling flow affecting cellular responses and maintaining healthy cellular states.
TRANK1 links to psychiatric conditions specifically bipolar disorder and schizophrenia. Research indicates aberrant expression or mutations impair normal brain functionality potentially disrupting emotion regulation and cognitive abilities. TRANK1 associates with proteins like DISC1 which are implicated in brain development and psychiatric disorders suggesting its involvement in neuronal signaling or synaptic functions. Understanding TRANK1 function in these contexts may provide insights into novel therapeutic strategies.