Abcam, ab259947, Anti-PHF19 antibody [EPR23919-31]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PHF19 antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23919-31,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IF, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHF19 often known as PHD Finger Protein 19 has important roles in chromatin remodeling by interacting with and regulating Polycomb Repressive Complex 2 (PRC2). This protein has a molecular mass approximately 74 kDa and expresses broadly in tissues with significant expression observed in proliferative and embryonic tissues. It contains PHD finger domains which lend it the ability to recognize specific histone modifications an important function in controlling gene expression.
Biological function summary
PHF19 plays a part in maintaining transcriptional repression of specific genes. It acts as an accessory component of the PRC2 complex which is responsible for catalyzing the tri-methylation of histone H3 on lysine 27 (H3K27me3) an important epigenetic mark for gene silencing. This interaction with PRC2 allows PHF19 to influence stem cell pluripotency and differentiation processes by targeting particular genomic loci for repression.
Pathways
PHF19 plays a role in the epigenetic regulation pathway by maintaining silenced gene states that affect the Wnt signaling pathway. This relationship ensures that developmental signals are tightly controlled. It collaborates with proteins like EZH2 a core component of PRC2 to affect these pathways. Furthermore PHF19 facilitates cross-talk with other chromatin-modifying proteins to reinforce the silencing effect.
PHF19 has connections to certain cancers such as prostate cancer and lymphoma where its disruption leads to deregulated expression of genes involved in cell cycle and proliferation. It affects these diseases through its modulation of EZH2's methyltransferase activity which becomes aberrant in these disorders leading to inappropriate gene silencing. PHF19's dysregulation also correlates with reactivation of oncogenes and loss of tumor suppressor gene silencing marking it as a significant factor in these pathological conditions.