Abcam, ab260053, Human Galectin-1 ELISA Kit

Size: 1 x 96Tests
Human Galectin-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Galectin-1 with a sensitivity of 0.36 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Serum, EDTA Plasma,
Reacts with:Mouse, Rat, Cow, Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.36 ng/mL,
Range:0.8 - 50 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Galectin-1 ELISA Kit (ab260053) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-1 protein in cell culture media, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Galectin-1 with 0.36 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Galectin-1, also known as 14 kDa lectin, galaptin, and lectin galactoside-binding soluble 1, is a β-galactoside binding lectin encoded by the gene LGALS1. Galectin-1 is a 134 amino acid non-glycosylated secreted protein that forms a non-covalently linked homodimer. Galectin-1 is expressed in a wide range of tissues and has various intra- and extra-cellular functions, where generally it is a modulator of immune responses. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.
Biological function summary
This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.
Pathways
The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.
Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.