Abcam, ab260055, Human GCP-2 ELISA Kit (CXCL6)

Size: 1 x 96Tests
Human GCP-2 ELISA Kit (CXCL6) is a single-wash 90-min Simplestep used to quantify Human GCP-2 (CXCL6) with a sensitivity of 13.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 13.1 pg/mL,
Range:25 - 1600 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human GCP-2 ELISA Kit (CXCL6) ab260055 is a rapid single-wash 90-min Sandwich ELISA to measure Human GCP-2 (CXCL6)in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 13.1 pg/mL.
How the assay works
Human GCP-2 (CXCL6)SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human GCP-2 ELISA Kit (CXCL6) ab260055 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-GCP2 antibody [EPR22310-215] - BSA and Azide free (Capture)
ab259523
- Anti-GRO gamma + GCP2 antibody [EPR22310-120] - BSA and Azide free (Detector)
ab259523
GCP-2 (Granulocyte chemotactic protein 2), also known as CXCL6 (C-X-C motif chemokine 6) is a chemotactic factor for neutrophil granulocytes. GCP-2 signals through binding and activation of its receptors CXCR1 and CXCR2. In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C