Abcam, ab263250, Human MEA1 knockout HEK-293T cell lysate

Size: 1Kit
MEA1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 14 bp insertion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 14 bp insertion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MEA1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MEA1 also known as Male-enhanced antigen 1 is a protein that plays a significant role in the process of spermatogenesis. It is a protein composed of approximately 65 kDa. MEA1 is predominantly expressed in the testes indicating its involvement in male reproductive function. The protein has been identified to participate in cellular processes essential to germ cell development and its expression pattern suggests it may have functions that are testis-specific or testis-enhanced.
Biological function summary
MEA1 interacts with cellular machinery involved in cell cycle regulation and differentiation. Its role has been observed in the development of male germ cells. MEA1 does not form part of a larger protein complex; instead it functions as an independent entity that supports spermatid differentiation. It seems essential for proper morphological changes necessary for mature sperm formation.
Pathways
Studies show that MEA1 is integral to pathways guiding spermatogenesis and germ cell maturation. It does not directly link to more widespread signaling routes like MAPK or PI3K. MEA1 may interact with proteins such as CREM a transcription factor that regulates genes critical for sperm cell development suggesting it integrates into the transcriptional regulatory framework essential for efficient sperm production.
MEA1’s aberrant expression is associated with male infertility specifically non-obstructive azoospermia which is a condition requiring intervention for sperm production failures. Connections to infertility emphasize the protein's role in maintaining healthy sperm production. Another protein associated with male infertility is DAZL which MEA1 could potentially interact with given their shared involvement in spermatogenesis. Further research could elucidate MEA1’s exact interactions and pathogenic roles connected to reproductive issues.