Abcam, ab263254, Human MPND knockout HeLa cell lysate

Size: 1Kit
MPND KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 7 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 7 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MPND, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MPND also known as Macrophage-derived Neutrophil Chemoattractant-related Protein has a molecular mass of approximately 41 kDa. It functions as an important component in immune cell signaling and activity. MPND is predominantly expressed in immune cells such as macrophages and neutrophils. It typically plays a role in innate immunity facilitating the recruitment and activation of immune cells during the inflammatory response.
Biological function summary
MPND affects several key processes within the immune system. It participates in immune cell communication and influences the chemotactic movement which is the ability of cells to move in response to chemical signals. MPND functions as part of a signaling complex that helps regulate inflammation by attracting neutrophils to sites of infection or injury ensuring a rapid immune response. In this context it interacts closely with other signaling molecules to modulate the immune system's effectiveness.
Pathways
MPND plays a significant role in the chemokine signaling pathway and the inflammatory response pathway. It interacts with proteins such as chemokine receptor CXCR2 and cytokines that facilitate cell signaling and immune cell migration. Through these pathways MPND functions as a bridge for communication between the immune cells translating extracellular signals into appropriate immune responses.
MPND stands out in conditions characterized by chronic inflammation and immune dysregulation. The protein has been linked to disorders such as rheumatoid arthritis and chronic obstructive pulmonary disease (COPD) where abnormal immune responses need regulation. In these diseases MPND interfaces with proteins such as TNF-alpha which also contribute to the inflammatory response making it an evolving target for therapeutic research aiming to control excessive inflammation.