Abcam, ab263260, Human MSH5 knockout HeLa cell lysate

Size: 1Kit
MSH5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 40 bp deletion in exon6.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 40 bp deletion in exon6.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MSH5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MutS Homolog 5 (MSH5) also known as human homolog of bacterial MutS plays a role in DNA repair and meiotic recombination. It is a protein composed of 834 amino acids with a molecular mass of approximately 93.7 kDa. MSH5 shows significant expression in reproductive tissues such as testes and ovaries indicating its importance in germ cell development. Other tissues like kidney and lung also express MSH5 suggesting roles beyond reproduction.
Biological function summary
MSH5 participates in maintaining genomic stability especially during meiosis. It forms a heterodimer with MSH4 creating a functional complex necessary for efficient crossing-over and recombination events. This complex recognizes and repairs mismatches and insertion-deletion loops ensuring genetic diversity during gamete formation. MSH5 also interacts with proteins involved in the DNA damage response linking its function to broader DNA repair mechanisms.
Pathways
MSH5 plays important roles in the meiotic recombination checkpoint and mismatch repair (MMR) pathways. In the meiotic recombination pathway MSH5 works alongside proteins like MLH1 and MLH3 ensuring successful homologous recombination. During the mismatch repair pathway the MSH4-MSH5 complex recognizes and binds to DNA mismatches recruiting other repair proteins to resolve these issues preventing mutations that can affect genomic integrity.
Mutations or altered expression of MSH5 have associations with infertility and certain cancers such as testicular cancer. The MSH5 alterations might disrupt the repair of DNA mismatches or meiotic recombination leading to genetic instability. In cancer the MSH5 protein might exhibit abnormal interactions with other repair proteins like BRCA1 compromising DNA repair processes and facilitating cancer progression. Understanding MSH5's role could advance our knowledge on genetic disorders related to recombination failures and guide potential therapeutic strategies.