Abcam, ab263280, Human NME6 knockout HEK-293T cell lysate

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NME6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3 and 8 bp deletion in exon3.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3 and 8 bp deletion in exon3.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NME6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NME6 also known as NM23-H6 or NDP kinase H6 is an enzyme that plays a role as a nucleoside diphosphate kinase. It weighs approximately 19 kDa and is part of the NME/NM23 nucleoside diphosphate kinase gene family. NME6 appears in various tissues with notable expression in the heart and skeletal muscle indicating its importance in tissues with high energy demands.
Biological function summary
NME6 functions in regulating the levels of nucleoside triphosphates within cells. The enzyme does not act alone; it associates as part of a larger complex where its activity supplements cellular energy metabolism. This involvement in energy conversion underlines its broader role in maintaining the balance of ATP and GTP concentrations which are essential for numerous cellular processes including signal transduction and cell proliferation.
Pathways
The enzyme NME6 integrates into ATP synthesis and metabolism pathways. It holds a relationship with the ATP citrate lyase pathway facilitating the interplay of energy production and consumption. Through this connection NME6 interacts with proteins such as NME1 and NME2 which also participate in maintaining cellular energetic homeostasis.
Alterations in the NME6 function have associations with mitochondrial disorders particularly those impacting tissues with high energetic needs. The enzyme also relates to cardiovascular diseases due to its expression in the cardiac muscle. Defective NME6 expression or function might cause disruptions in energy homeostasis leading to disease states. Additionally through these diseases NME6 potentially connects to other proteins involved in energy metabolism like ATP synthase.