Abcam, ab263298, Human PIP5K1A knockout HEK-293T cell lysate

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PIP5K1A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PIP5K1A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIP5K1 alpha also known as Phosphatidylinositol-4-phosphate 5-kinase type 1 alpha (PIP5K1A) is an enzyme that catalyzes the phosphorylation of phosphatidylinositol-4-phosphate (PI4P) to generate phosphatidylinositol-45-bisphosphate (PI(45)P2). The protein has an approximate mass of 68 kDa and is highly expressed in a variety of human tissues including the brain heart and spleen. PIP5K1A plays a significant role in lipid signaling and membrane dynamics impacting a wide array of cellular processes such as cytoskeletal organization and vesicle trafficking.
Biological function summary
PIP5K1A influences the production of PI(45)P2 an important phosphoinositide involved in numerous cellular functions. PI(45)P2 acts as a substrate for the production of secondary messengers like inositol trisphosphate (IP3) and diacylglycerol (DAG) essential in signal transduction pathways. PIP5K1A is part of a larger protein complex interacting with cytoskeletal elements and regulators of endocytosis and exocytosis. This association highlights its importance in maintaining cellular compartmentalization and function.
Pathways
PIP5K1A plays a pivotal role in the phosphoinositide signaling pathway and the actin cytoskeleton regulation pathway. It works closely with proteins such as RAC1 a small GTPase involved in actin polymerization to facilitate changes in the cell structure and motility. Additionally PIP5K1A's product PI(45)P2 serves as a precursor for multiple signaling molecules integrating with pathways that mediate cellular responses to various extracellular stimuli.
PIP5K1A's dysregulation links to cancer and neurological disorders. In cancer alterations in PIP5K1A expression or activity can affect cell proliferation and survival interacting with proteins like AKT that drive oncogenic signaling. In the context of neurological disorders its role in synaptic function and plasticity directly connects to conditions like schizophrenia where PI(45)P2 imbalance may contribute to the disorder's pathophysiology. These associations highlight the essential impact of PIP5K1A in maintaining cellular homeostasis and its potential as a therapeutic target.