Abcam, ab263312, Human PPP4R2 knockout HEK-293T cell lysate

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PPP4R2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 1 bp insertion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 1 bp insertion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PPP4R2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein phosphatase 4 regulatory subunit 2 commonly known as PPP4R2 serves as a regulatory subunit in the protein phosphatase 4 (PP4) complex. It has an approximate mass of 50 kDa. PPP4R2 is mainly expressed in the cytoplasm of cells where it regulates the activity and specificity of PP4. It is involved in various cellular processes due to its capacity to modulate PP4's activity.
Biological function summary
PPP4R2 plays a significant role in DNA damage repair processes. PPP4R2 is part of the PP4 complex and together with other components it oversees dephosphorylation processes post DNA damage which is essential for cell cycle progression and genomic stability. It collaborates with other proteins in the complex to ensure accurate DNA repair maintaining proper cellular function and preventing mutations.
Pathways
PPP4R2 is notably involved in the DNA repair pathway and cell cycle regulation pathway. These pathways are critical for maintaining cell integrity and viability. Within the DNA repair pathway PPP4R2 operates closely with proteins like PP4C the catalytic subunit of PP4 which performs dephosphorylation tasks essential for DNA damage response. In the cell cycle regulation pathway PPP4R2 ensures appropriate cell cycle checkpoints by interacting with multiple cellular proteins which helps in maintaining genomic stability.
Research links PPP4R2 to cancer and neurodegenerative diseases highlighting its importance in maintaining genomic integrity. In cancer alterations in PPP4R2 expression or function can affect DNA repair leading to genomic instability and tumorigenesis. PPP4R2 interacts with proteins such as CHK2 that are important in the DNA damage response. Moreover abnormal PPP4R2 activity impacts neurodegenerative conditions where defects in DNA repair mechanisms are implicated. Understanding its role in these contexts aids in potential therapeutic targeting strategies.