Abcam, ab263317, Human PSRC1 (DDA3) knockout HeLa cell lysate

Size: 1Kit
PSRC1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PSRC1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PSRC1 protein also known as DDA3 plays an important role in cell cycle regulation. It is a microtubule-associated protein with a mass of approximately 33 kDa. PSRC1 is widely expressed in various tissues including the brain heart and liver. It interacts directly with microtubule plus-end tracking proteins and regulates microtubule dynamics which is essential for proper mitotic spindle function.
Biological function summary
PSRC1 plays an important role in ensuring correct chromosome segregation during cell division. It functions as part of the mitotic spindle assembly complex and is responsible for promoting stable attachment of microtubules to kinetochores which is critical for accurate chromosomal alignment. These functions involve interactions with proteins related to spindle formation and checkpoint signaling.
Pathways
PSRC1 acts in the mitotic spindle checkpoint pathway and the microtubule dynamics pathway. Its interaction with proteins like CLASP1 and APC important for microtubule stability and dynamics highlights its contribution to maintaining proper mitotic progression. PSRC1 ensures successful completion of mitosis by coordinating these pathways important for cell division fidelity.
PSRC1 connects to various types of cancer such as breast and colorectal cancer. It influences tumor progression through its effect on cell division errors and genomic instability. Additionally PSRC1 interacts with TP53 a protein responsible for cell cycle regulation and apoptosis suggesting its potential role in tumor suppression mechanisms compromised in cancerous conditions.