Abcam, ab263345, Human SHKBP1 knockout HeLa cell lysate

Size: 1Kit
SHKBP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SHKBP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SHKBP1 also known as Sh3kbp1-binding protein 1 is a protein that plays an influential mechanical role in cellular processes such as signal transduction. It has a molecular mass of approximately 76 kDa. This protein is expressed in various tissues with notable abundance in the brain and immune-related cells. As a part of its mechanical function SHKBP1 interacts with components of several signaling pathways helping to regulate cellular responses to external stimuli.
Biological function summary
SHKBP1 is engaged in the modulation of signal transduction and cell communication. It participates in molecular complexes that are involved in the regulation of endocytosis and intracellular trafficking. SHKBP1 interacts with proteins like Cbl which plays a significant role in mediating ubiquitin-protein ligase activities. This interaction can influence how receptors and proteins are marked for degradation affecting cellular communication networks.
Pathways
The protein SHKBP1 integrates into signaling routes such as the ubiquitin-proteasome pathway and the signaling cascades involving Cbl proteins. These pathways govern critical cell cycle events and protein stability. SHKBP1 forms part of the complex machinery that manages the sorting and degradation of receptor tyrosine kinases participating in processes alongside other related proteins like Grb2. This involvement highlights its significant role within the larger framework of cell signaling dynamics.
Abnormalities in SHKBP1 expression or function connect with the development of cancer and neurodegenerative diseases. For example its altered regulatory role in ubiquitination can lead to unrestrained cell proliferation contributing to oncogenesis. Moreover disruptions in signaling pathways where SHKBP1 cooperates with Cbl and other binding partners could relate to neural tissue damage implicating SHKBP1 in neurodegenerative conditions including Alzheimer's disease.