Abcam, ab263357, Human SLC52A2 (GPR172A/PAR1) knockout HeLa cell lysate

Size: 1Kit
SLC52A2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC52A2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GPR172A also known as PAR1 is a G protein-coupled receptor with roles in various cellular processes. Its molecular mass is approximately 48 kDa. This protein is expressed in many tissues including the liver kidney and brain. As a receptor it interacts with G proteins to transmit signals from the outside of the cell to the inside influencing the cell's response to external stimuli.
Biological function summary
GPR172A/PAR1 plays an important role in regulating blood clotting and inflammation. It is part of a signaling complex that includes thrombin a serine protease. This complex is important for activating platelets which are cells involved in the blood clotting process. By activating platelets GPR172A/PAR1 helps control bleeding and wound healing.
Pathways
The protein is involved in both the coagulation and inflammatory pathways. In the coagulation pathway it works alongside other proteins like thrombin and fibrinogen contributing to clot formation. In inflammation GPR172A/PAR1 interacts with proteins such as NF-kB helping to regulate inflammatory responses and cytokine production which are important for immune defense and tissue repair.
GPR172A/PAR1 has a connection to cardiovascular diseases and inflammatory disorders. Its role in promoting blood clot formation links it to conditions like thrombosis where abnormal clotting can lead to dangerous blockages in blood vessels. GPR172A/PAR1 also relates to inflammatory disorders through its interaction with inflammatory proteins such as NF-kB. Abnormal activity of this receptor can lead to excessive inflammation contributing to diseases like rheumatoid arthritis.