Abcam, ab263366, Human SSBP4 knockout HEK-293T cell lysate

Size: 1Kit
SSBP4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SSBP4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SSBP4 also known as single-stranded DNA-binding protein 4 plays a role in DNA metabolic processes. It binds single-stranded DNA and assists in maintaining DNA stability during replication and repair. SSBP4 has an approximate molecular weight of 35 kDa. This protein is expressed in various tissues including the brain liver spleen and kidney contributing to its versatile functions in the human body.
Biological function summary
Single-stranded binding proteins including SSBP4 participate in managing genome stability by shielding DNA strands during replication. SSBP4 acts as a safeguard against DNA damage by preventing strand re-hybridization. Although SSBP4 operates independently its function is important in the complex machinery of DNA replication and repair.
Pathways
SSBP4 supports DNA replication and repair pathways often working alongside homologous proteins such as RPA (replication protein A). These pathways ensure accurate replication of the genome and help maintain genetic integrity during cell division. SSBP4's role in these pathways involves coordinating with other replication machinery to facilitate unwinding and protection of DNA strands.
Alterations in SSBP4 activity may be implicated in cancer particularly those involving genomic instability. Irregularities in DNA repair processes contribute to tumorigenesis making SSBP4 a potential focus for cancer research. Furthermore SSBP4 shares pathway connections with another involved protein BRCA2 which is also known for its role in maintaining DNA stability and is linked to hereditary breast and ovarian cancer.