Abcam, ab263368, Human SSSCA1 knockout HEK-293T cell lysate

Size: 1Kit
ZNRD2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ZNRD2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SSSCA1 also known as spindle and kinetochore-associated complex subunit 1 is a protein with a molecular mass of approximately 11 kDa. It plays a significant role in cell division. SSSCA1 localizes to the nucleus and associates with the mitotic spindle during cell cycle progression. Expression of SSSCA1 can vary across different tissues but it is particularly prominent in rapidly dividing cells including those in the testis and thymus.
Biological function summary
The protein acts as a critical player in the regulation of mitosis. SSSCA1 forms part of a larger multiprotein complex essential for the proper attachment of chromosomes to the mitotic spindle. By ensuring that the chromosomes align correctly SSSCA1 facilitates accurate segregation during cell division. It works closely with other components of the complex to maintain genomic stability.
Pathways
The protein influences critical cell division processes. SSSCA1 integrates into the spindle assembly checkpoint pathway ensuring the proper timing of mitosis. Within this pathway it often interacts with proteins such as BubR1 and Mad2 which are responsible for halting cell cycle progression until chromosomes are correctly attached to the spindle fibers. Its role in checkpoint pathways highlights its importance in maintaining orderly mitotic progression.
Defects in the expression or function of SSSCA1 have connections to cancer development. Cancer cells frequently exhibit abnormal cell division and dysregulation of SSSCA1 can exacerbate these issues. The protein's interaction with p53 a well-known tumor suppressor protein might further hint at its relevance to cancer proliferation. Additionally anomalies in the functioning of SSSCA1 have also been observed in various chromosomal instability syndromes highlighting its influence in maintaining chromosome integrity.