Abcam, ab263371, Human STK40 knockout HeLa cell lysate

Size: 1Kit
STK40 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-STK40, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
STK40 also known as Serine/Threonine Kinase 40 or protein-cancelled testis is an important kinase involved in several cellular processes. This protein has a mass of approximately 52 kDa. STK40 is mainly expressed in embryonic tissues and adult testis but also observed in many other tissues highlighting its various roles during development and in adult organisms. STK40 functions by phosphorylating target proteins which affects their function and regulates their activities within the cell.
Biological function summary
STK40 influences cell differentiation and development. It forms part of signaling complexes interacting with various other proteins to impart its effect. STK40 influences pluripotency in stem cells by modulating transcription factors and signaling pathways critical for maintaining a stable differentiation state. Moreover STK40 is shown to play a role in adipogenesis by affecting pathways that control fat cell formation and distribution.
Pathways
STK40 functions actively within several signaling cascades notably the MAPK and Wnt pathways. In the MAPK pathway it coordinates with proteins like MAP3K7 to control transcriptional activation linked to cellular stress responses and differentiation processes. In the Wnt signaling pathway STK40 influences the stabilization of beta-catenin a central protein in regulating gene expression related to cell proliferation and fate decisions.
STK40 has connections to cancer and metabolic syndromes. Dysregulation of STK40 expression or function can lead to improper cell differentiation and proliferation contributing to tumorigenesis particularly in testicular cancer. In metabolic disorders such as obesity STK40’s role in adipogenesis can affect fat deposition and might interlink with other proteins like PPARγ which further influences lipid metabolism and insulin sensitivity. Understanding STK40’s role in these conditions highlights its potential as a therapeutic target.