Abcam, ab263378, Human SYNGR2 (Synaptogyrin 2) knockout HEK-293T cell lysate

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SYNGR2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 208 bp deletion in exon1 and 44 bp deletion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 208 bp deletion in exon1 and 44 bp deletion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SYNGR2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Synaptogyrin 2 also known as SYNGR2 is a membrane protein with a known molecular weight of approximately 26.5 kDa. It is a component of the synaptogyrin family which consists of proteins located in neuronal and endocrine tissues. Synaptogyrin 2 is a tetraspan membrane protein found in the vesicles of presynaptic neurons where it plays a role in modulating neurotransmitter release at the synapse. Its presence is prominent in the brain as well as in other nerve tissues.
Biological function summary
This protein contributes to the regulation of synaptic transmission. Synaptogyrin 2 does not act alone; instead it interacts with other proteins forming a complex that helps in managing the availability and recycling of synaptic vesicles. This regulation is significant for maintaining neurotransmitter homeostasis in neural communication influencing synaptic plasticity and overall brain function.
Pathways
The function of Synaptogyrin 2 relates to the synaptic vesicle cycle and the neurotransmitter release pathway. It closely associates with proteins like synaptophysin and synaptotagmin which are essential for the process of synaptic vesicle exocytosis and endocytosis. Through these pathways Synaptogyrin 2 impacts the efficiency and fidelity of neurotransmission which are critical features of neuronal communication.
Impairments involving Synaptogyrin 2 affect neurological functions linking it to disorders such as schizophrenia and epilepsy. Its interaction with proteins like SNAP-25 and syntaxin emphasizes its role in ensuring proper synaptic transmission. Abnormalities in these interactions may lead to altered synaptic function contributing to the pathophysiology of these conditions. Understanding Synaptogyrin 2’s role in these disorders aids in developing targeted therapeutic approaches.