Abcam, ab263379, Human TACC2 knockout HeLa cell lysate

Size: 1Kit
TACC2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 9.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 9.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TACC2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TACC2 also known as Transforming Acidic Coiled-Coil-Containing Protein 2 is a protein involved in cell cycle regulation. It has a molecular mass of approximately 146 kDa and is expressed in various tissues with notable expression in the brain. TACC2 interacts with microtubules stabilizing their structure which is essential for proper mitotic spindle formation during cell division. This stabilization facilitates accurate chromosome segregation a critical aspect of cellular replication.
Biological function summary
TACC2 plays a role in centrosome-related processes and is part of a multi-protein complex involved in microtubule dynamics. The protein is particularly important in neuronal cells where it contributes to maintaining proper cell architecture and neurogenesis. Studies have shown that TACC2 interacts with components involved in chromosomal stability ensuring the faithful transmission of genetic information during cell division thereby supporting cellular homeostasis.
Pathways
TACC2 is involved in cellular pathways such as the mitotic spindle assembly checkpoint and the Aurora kinase signaling pathway. It directly interacts with Aurora A a kinase that regulates mitotic entry and centrosome maturation. Aurora A works closely with TACC2 to promote the correct formation of the mitotic apparatus ensuring successful chromosomal alignment and segregation during cell division. This cooperation is essential to maintain genomic stability and prevent aneuploidy.
TACC2 relates to cancer and neurodevelopmental disorders. Abnormal expression or mutations in TACC2 are linked to certain cancer types often involving incomplete cytokinesis due to disrupted mitotic spindle function. It also interacts with proteins like Aurora A in these contexts which can contribute to oncogenic pathways. Furthermore alterations in TACC2 function are associated with neurodevelopmental disorders highlighting its critical role in neural tissue where failure to regulate microtubule dynamics can result in developmental abnormalities.