Abcam, ab263385, Human THOC3 knockout HeLa cell lysate

Size: 1Kit
THOC3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-THOC3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
THOC3 also known as THO complex subunit 3 is a component of the mRNA-export complex that plays an important role in RNA processing. It has a molecular mass of approximately 35 kDa. THOC3 is mainly expressed in the nucleus where it associates with other subunits to form the TREX complex which is essential for loading and export of mRNA transcripts. This protein ensures smooth mRNA transport from the nucleus to the cytoplasm influencing gene expression dynamics.
Biological function summary
THOC3 functions as part of the TREX complex which couples transcription with mRNA processing and export. The TREX complex is responsible for enhancing the efficiency of transcription elongation and mRNA release enabling the proper export of mRNA to the cytoplasm. THOC3 along with other core components of the complex maintains the integrity of mRNA ensuring only correctly processed mRNA exits the nucleus for translation.
Pathways
THOC3 is a critical participant in the mRNA export pathway an important aspect of gene expression regulation. This protein interacts with other members of the TREX complex such as THOC1 and THOC2 facilitating mRNA export and ensuring seamless communication between transcription and translation processes. Besides THOC3 ties into the larger gene expression and regulation network supporting cellular growth and function.
THOC3 has links to conditions involving cellular proliferation and gene expression dysregulation including certain cancers. Abnormal function or expression of THOC3 can disrupt normal mRNA processing and export contributing to oncogenesis. Altered interactions with related proteins such as ALYREF another mRNA export component can further influence disease progression. Understanding THOC3’s role in these disorders offers potential for targeted therapeutic approaches.