Abcam, ab263423, Human WSB2 knockout HeLa cell lysate

Size: 1Kit
WSB2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6 and 2 bp insertion in exon6.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6 and 2 bp insertion in exon6.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-WSB2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WSB2 also known as WD Repeat and SOCS Box-containing protein 2 weighs approximately 38 kDa. Mechanically WSB2 supports protein degradation and regulates ubiquitination. It presents ubiquitin ligase activity through interaction with other proteins marking them for degradation. You can find WSB2 widely expressed in various tissues including the brain kidney and heart.
Biological function summary
WSB2 plays a role in cellular processes related to protein homeostasis. It is part of a larger protein ubiquitination complex that includes other SOCS box-containing proteins. This complex facilitates targeted degradation of substrates contributing to cellular quality control. WSB2 influences cellular functions by managing levels of important regulatory proteins.
Pathways
WSB2 impacts processes like the JAK-STAT signaling and Wnt signaling pathways. In the JAK-STAT pathway WSB2 regulates signaling by modulating degradation of pathway components. It maintains cellular responses by ensuring the precise level and turnover of these proteins. Through these actions WSB2 has relationship with proteins like JAKs and KIT affecting cellular growth and communication.
WSB2 connects with cancer and neurological disorders. Dysregulation of its ubiquitination activity relates to tumor progression in cancers impacting proteins such as p53. In neurological contexts WSB2 may be involved in neurodegenerative disorders through its effect on protein aggregates. Its interaction with proteins involved in these pathways and conditions suggests potential relevance in disease mechanisms.