Abcam, ab263427, Human YY1AP1 knockout HeLa cell lysate

Size: 1Kit
YY1AP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-YY1AP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
YY1AP1 also known as Yin Yang 1-Associated Protein 1 has a molecular weight of approximately 75 kDa. It mainly interacts with the YY1 transcription factor modulating transcriptional activity. Researchers find YY1AP1 expressed in various tissues with higher levels noted in the heart brain and liver. The protein localizes itself in both the nucleus and cytoplasm.
Biological function summary
YY1AP1 affects gene regulation and chromatin remodeling. It forms part of a transcriptional complex that includes YY1 and other cofactors. This complex impacts processes like cell proliferation and differentiation through manipulating transcription. YY1AP1 holds a strong role in influencing cellular homeostasis affecting development and cellular response to stress.
Pathways
YY1AP1 plays a role in pathways involving transcriptional regulation and chromatin dynamics. It participates in the Wnt signaling pathway and the p53 pathway. Within these pathways YY1AP1 associates with proteins like β-catenin and Mdm2 aiding in regulating cell cycle and apoptosis. The interactions show YY1AP1's importance in signal transduction and transcriptional response mechanisms.
YY1AP1 connects to cancer and congenital heart disease. In cancer its interaction with YY1 and other transcription factors suggests a role in tumorigenesis where it affects cell cycle regulation and apoptosis. In congenital heart disease altered expression patterns of YY1AP1 or disruption of its interaction with key proteins like GATA4 could contribute to developmental defects. Understanding YY1AP1's involvement in these conditions might offer insight into therapeutic targets and mechanisms.