Abcam, ab263429, Human ZC3H15 knockout HEK-293T cell lysate

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ZC3H15 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ZC3H15, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Zinc finger CCCH-type containing 15 (ZC3H15) is a small protein with a mass of approximately 70 kDa. The protein has an additional name NDP52 that arises from research in specific contexts of cell biology. ZC3H15 contains a zinc finger domain that binds RNA indicating its role in post-transcriptional regulation. The protein shows expression in multiple cell types but higher expression levels occur in immune cells reflecting its specialized functions in immunity and cellular signaling.
Biological function summary
ZC3H15 participates in the regulation of mRNA stability and degradation impacting gene expression dynamically. The protein acts as part of a ribonucleoprotein complex working together with other proteins to influence RNA processing events. ZC3H15 modulates innate immune responses by controlling the degradation and translation of mRNAs encoding inflammatory mediators. This regulation helps maintain cellular homeostasis during immune challenges.
Pathways
ZC3H15 plays significant roles in the NF-kB signaling and apoptosis pathways. It influences the NF-kB pathway by interacting with proteins that control inflammatory gene expression such as TRAF2 and TAK1. ZC3H15 affects the apoptotic pathway through its impact on mRNA stability altering the expression of key apoptotic regulators. These interactions showcase its functional importance in balancing cell survival and death decisions under stress and pathological conditions.
ZC3H15 has associations with autoimmune conditions and infectious diseases. Dysregulation of ZC3H15 can lead to excessive inflammation contributing to diseases like rheumatoid arthritis through mishandled NF-kB signaling. In infectious diseases the modulation of ZC3H15 expression links to viral pathology as certain viruses exploit this protein to evade immune responses. Exploring these connections could inform potential therapies targeting ZC3H15 or its associated pathways involving proteins like MAVS that play central roles in antiviral defense.