Abcam, ab263433, Human ZNF609 knockout HeLa cell lysate

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ZNF609 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ZNF609, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZNF609 also known as Zinc Finger Protein 609 functions as a transcription factor. It belongs to the Krüppel-associated box (KRAB) zinc finger protein family which consists of proteins important in gene regulation. The molecular mass of ZNF609 is approximately 177 kDa. It expresses in several tissues with higher levels found in embryonic tissues and some adult brain areas highlighting its significance during development.
Biological function summary
ZNF609 participates in regulating gene expression by binding to specific DNA sequences. It is not known to be part of a complex but often interacts with other transcription factors and co-regulators. Its regulatory function can activate or repress various downstream genes thereby impacting cellular growth and differentiation processes. The protein plays essential roles in neurodevelopment and muscle tissue formation contributing to normal growth and function.
Pathways
ZNF609 interfaces closely with the Wnt signaling pathway which is essential for cell fate determination and embryogenesis. It also influences the myogenesis regulatory pathway partnering with other proteins like MYOD1 to direct muscle differentiation. These interactions situate ZNF609 in a critical part of the cellular machinery ensuring proper growth and development.
ZNF609 has associations with neurodevelopmental disorders and certain muscular dystrophies. Disruption or mutation of this protein has links to intellectual disability due to its significant role in brain development. In muscular conditions such as congenital myopathies ZNF609 interacts with proteins like DMD (dystrophin) where abnormal function can result in muscle weakness and degeneration. Understanding these connections is vital for therapeutic approaches targeting these conditions.