Abcam, ab263434, Human ZNHIT6 (C1orf181) knockout HeLa cell lysate

Size: 1Kit
ZNHIT6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 2 bp insertion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 2 bp insertion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ZNHIT6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C1orf181 also known as Chromosome 1 Open Reading Frame 181 is a relatively less characterized protein which has a calculated mass of approximately 22 kDa. This protein is expressed in multiple tissues but it shows higher expression in testis and brain suggesting potential roles in these organs. C1orf181 might have specific functions related to particular cellular processes although extensive studies on its precise mechanical role remain limited.
Biological function summary
C1orf181 is associated with cellular processes that impact gene regulation and protein interactions. Although not much is known about its role evidence suggests it might be involved in protein complexes that contribute to maintaining cellular homeostasis. The structure or partners in these complexes have not been fully identified but ongoing research is aiming to unveil its interacting partners and biological implications.
Pathways
The involvement of C1orf181 may connect to regulatory networks influencing cell growth and signaling. Studies indicate potential association with the MAPK signaling pathway an important mechanism in cell proliferation and differentiation. Additionally interactions with proteins like ERK1/2 which are vital components of this pathway point towards C1orf181 influencing downstream cellular responses.
Research demonstrates some connection between C1orf181 and neurological or reproductive health issues given its expression patterns. Alterations in the function or expression of C1orf181 might associate with conditions like Parkinson's Disease or testicular dysfunctions. Researchers are examining potential interactions with proteins such as alpha-synuclein in Parkinson's to ascertain its role in disease pathology. Further studies are necessary to fully elucidate these links and their therapeutic relevance.