Abcam, ab263877, Human NT-proBNP ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human NT-proBNP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NT-proBNP with a sensitivity of 11.5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 11.5 pg/mL,
Range:21.9 - 1400 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human NT-proBNP ELISA Kit ab263877 is a rapid single-wash 90-min sandwich ELISA kit to measure Human NT-proBNP in Serum, and Plasma (Citrate, EDTA, Heparin). This Simplestep sensitivity is 11.5 pg/mL.
How the assay works
Human NT-proBNP SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
The antibodies in this assay were found not to react with the BNP-32 peptide sequence.
Human NT-proBNP ELISA Kit ab263877 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
How other researchers are using our Human NT-proBNP ELISA Kit ab263877
Human NT-proBNP ELISA Kit ab263877 has been used to study diabetes
and heart disease
References:
U. Umar, et.al., 2023, PMID: 37513325
S. Xie, et.al., 2023, PMID: 37492291
Related and Recommended products
Human NT-proBNP ELISA Kit ab263877 is commonly used to measure NT-proBNP as a marker of cardiotoxicity.
Other ELISA kits to measure cardiotoxicity biomarkers include:
- Human NT-ProANP ELISA Kit
ab245705
- Human Cardiac Troponin I ELISA Kit
ab200016
- Human Cardiac Troponin T ELISA Kit
ab223860
- Human MYBPC3 ELISA Kit
ab323737
NT-proBNP, or Brain Natriuretic Peptide, is a hormone expressed by cardiomyocytes by the gene
. NT-proBNP is synthesized as a 134- amino acid preprohormone (prepro-BNP), subsequent removal of a 25 amino acid signal peptide forms pro-BNP, which is stored intracellularly. Pro-BNP is secreted as an inactive prohormone and cleavage by furin or corin forms NT-pro-BNP and the active 32- amino acid BNP-32 peptide. BNP may function as a paracrine antifibrotic factor in the heart, as well as have a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. The antibody pair in this product detect NT-pro-BNP.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NT-proBNP known as N-terminal prohormone of brain natriuretic peptide is a non-active prohormone that measures approximately 8.5 kDa in mass. This prohormone emerges from the cleavage of pro-BNP in cardiomyocytes specifically in the heart ventricles. NT-proBNP levels rise in response to ventricular volume expansion and pressure overload therefore serving as an important indicator of cardiac function. Researchers regularly utilize assays like NT-proBNP ELISA and NT-proBNP monoclonal antibodies for detecting its presence in various biological samples.
Biological function summary
NT-proBNP stands important for cardiovascular homeostasis. It does not participate as part of a complex but plays a role on its own. When released into circulation NT-proBNP functions as an indicator of heart performance indirectly reflecting the activity of the active hormone BNP. Blood vessels dilate under BNP influence reducing systemic vascular resistance and central venous pressure which aids in heart function improvement.
Pathways
NT-proBNP interacts significantly within the cardiac stress response pathways. It plays a focus within the natriuretic peptide system interacting with proteins such as BNP and ANP that help regulate fluid balance and blood pressure. These components together influence pathways that modulate hemodynamic stress and sodium excretion ensuring cardiovascular stability under stress conditions.
Clinicians use NT-proBNP as a biomarker for heart failure and ischemic heart disease. Elevated levels of NT-proBNP correlate with cardiac stress indicating unmet cardiac workload or myocardial ischemia. In these contexts NT-proBNP serves alongside BNP as an indicator of increased heart strain thereby offering insights into disease progression and patient response to therapeutic interventions.