Abcam, ab263880, Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone)

Size: 1 x 96Tests / 10 x 96Tests
Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) is a single-wash 90-min Simplestep used to quantify Mouse/Rat ACTH (adrenocorticotropic hormone) with a sensitivity of 6 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 10 citations
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Mouse, Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 6 pg/mL,
Range:7.81 - 500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) ab263880 is a rapid single-wash 90-min ELISA kit to measure Mouse/Rat ACTH in serum and plasma. This SimpleStep ELISA® sensitivity is 6 pg/mL.
How the assay works
Mouse/Rat ACTH SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes native mouse and rat ACTH in serum and plasma samples only. As ACTH is a product of POMC, this kit is expected to cross-react in any samples that contain the unprocessed precursor. Cell culture supernatant, cell and tissue extract samples have not been tested with this kit.
Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) ab263880 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse/Rat ACTH ELISA kit ab263880
Mouse/Rat ACTH ELISA kit ab263880 has been used to measure the hypothalamus–pituitary–adrenal axis activation
(1)(2)
References:
Hoyoung Kim et al. 2023, PMID: 37653040,
Weiyi Chen et al. 2023, PMID: 37075752
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse/Rat ACTH (adrenocorticotropic hormone) Antibody Pair - BSA and Azide free
ab267700
Related and recommended products
Mouse/Rat ELISA kit ab263880 is commonly used to measure the activity of the hypothalamic-pituitary-adrenal axis.
Other ELISA kits to measure related hormones include:
- Cortisol ELISA kit
ab108665
- Human Growth Hormone ELISA kit
ab190811
ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. ACTH's primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones. The peptide sequence for this hormone is highly evolutionarily conserved amongst mammals. Specifically, for mouse and rat, this sequence shares 100% identity.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C