Abcam, ab263881, Human AST ELISA Kit (Aspartate Aminotransferase)

Size: 1 x 96Tests / 1 x 384Tests
Human AST ELISA Kit (Aspartate Aminotransferase) is a single-wash 90-min Simplestep used to quantify Human AST (Aspartate Aminotransferase) with a sensitivity of 43 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 43 pg/mL,
Range:312.5 - 20000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 is a rapid single-wash 90-min ELISA kit to measure Human AST in cell culture extracts, tissue extracts, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 43 pg/mL.
How the assay works
Human AST SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of human AST. The standard protein in this kit is full length human AST. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin), cell culture supernatant, cell extract and tissue extract sample types. Saliva, urine, milk, and CSF samples have not been tested with this kit.
Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human AST ELISA Kit (Aspartate Aminotransferase) ab263881
Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 has been used to study oxidative and inflammatory responses to smoking
, blood biomarker expression in acute pancreatitis
, and human Liver-Chip as a model for predicting drug-induced liver injury
References:
Hande Sipahi et al. 2023, PMID: 36650104,
Huaming Ding et al. 2022, PMID: 36816737,
Janey Ronxhi, et al. 2019, PMID: 35790095
Save your precious samples
Our 384-well Human AST ELISA Kit (Aspartate Aminotransferase) only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Rat Aspartate Aminotransferase Antibody Pair - BSA and Azide free
ab267701
- Anti-Aspartate Aminotransferase antibody [EPR20781-274] - BSA and Azide free (Capture)
ab259691
- Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector)
ab259692
Related and recommended products
Human AST ELISA Kit (Aspartate Aminotransferase) ab263881 is commonly used by researchers to measure liver function.
Other ELISA kits to measure liver function include:
- Human ALT ELISA kit
ab234578
- Human Albumin ELISA kit
ab179887
- Albumin (BCG) Assay Kit (Colorimetric)
ab235628
These ELISA kits are also available for Mouse/Rat:
- Mouse AST ELISA kit
ab263882
- Mouse ALT ELISA kit
ab282882
- Mouse Albumin ELISA kit
ab207620
- Rat AST ELISA kit
ab263883
- Rat ALT ELISA kit abab234579
- Rat Albumin ELISA kit
ab235642
Cytoplasmic aspartate aminotransaminase (AST), is a key enzyme in the regulation of glutamate levels. It catalyzes the reversible transfer of an a-amino group between aspartate and glutamate. Given its central role in amino acid metabolism, it plays a critical role in many biological functions. In a clinical context, AST levels in the serum are used as a marker for liver health. This kit targets the full-length protein.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C