Abcam, ab263882, Mouse AST ELISA Kit (Aspartate Aminotransferase)

Size: 1 x 96Tests / 1 x 384Tests
Mouse AST ELISA kit is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of mouse aspartate aminotransferase (AST/cAspAT) with a sensitivity of 39 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader - Available in different formats to meet various throughput needs, including 384-well plates for higher throughput - Design your own immunoassay: we also offer the conjugation-ready, carrier-dree antibody pair. - Cited in over 25 publications
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 39 pg/mL,
Range:125 - 8000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse AST ELISA Kit (Aspartate Aminotransferase) ab263882 is a rapid single-wash 90-min ELISA kit to measure tissue lysate, cell lysate, serum and plasma. This SimpleStep ELISA® sensitivity is 39 pg/mL.
How the assay works
SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of mouse AST. The standard protein in this kit is full length mouse AST. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin), cell extract, and tissue extract sample types. Cell culture supernatant, urine, saliva, milk, and CSF samples have not been tested with this kit.
Recombinant Human IL-1 beta, Mouse IL-1 alpha, and Mouse IL-1 receptor1 were prepared at 5 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
Recombinant Human IL-1 beta, Mouse IL-1 alpha, and Mouse IL-1 receptor1 were prepared at 5 ng/mL and tested for interference. No interference with was observed.
Mouse AST ELISA Kit (Aspartate Aminotransferase) ab263882 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse AST ELISA Kit ab263882
Mouse AST ELISA Kit ab263882 has been used to measure AST plasma to study mitochondrial homeostatis in liver disease
and rencofilstat as a treatment for HCV-induced hepatocellular carcinoma
References:
Ai-Lei Xu et al. 2024, PMID: 37902450,
Philippe Gallay et al. 2023, PMID: 37896876
Save your precious samples
Our 384-well Mouse AST ELISA Kit (Aspartate Aminotransferase) only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse/Rat Aspartate Aminotransferase Antibody Pair - BSA and Azide free
ab267702
- Anti-Aspartate Aminotransferase antibody [EPR20781-22] - BSA and Azide free (Capture)
ab259695
- Anti-Aspartate Aminotransferase antibody [EPR22206-177] - BSA and Azide free (Detector)
ab259696
Related and recommended products
Mouse AST ELISA Kit ab263882 is commonly used by researchers to measure liver function.
Other ELISA kits to measure liver function include:
- Mouse ALT ELISA kit
ab234578
- Mouse Albumin ELISA kit
ab282297
- Mouse Alkaline Phosphatase ELISA kit
ab285274
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
Biological function summary
AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.
Pathways
AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.
Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.