Abcam, ab264220, Anti-PRKAR2 antibody

Size: 100µL
Rabbit Polyclonal PKA R2/PKR2 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PRKAR2A aa 50-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PRKAR2A aa 50-100. The exact immunogen used to generate this antibody is proprietary information.P13861

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PRKAR2 protein also known as the protein kinase cAMP-dependent type II regulatory subunit beta plays an important role in regulating protein kinase A (PKA) activity. This protein has a molecular mass of approximately 46 kDa and is found mainly in human tissues such as the brain liver and heart. PRKAR2 forms a complex with catalytic subunits to modulate PKA's activity when cyclic AMP (cAMP) levels fluctuate. This intricate mechanism allows it to effectively control cellular responses through phosphorylation.
Biological function summary
Protein kinase A activity depends largely on the proper function of PRKAR2. PRKAR2 is part of the larger PKA holoenzyme complex which includes regulatory and catalytic subunits. PRKAR2 when bound to cAMP changes its conformation causing the dissociation of the catalytic subunits leading to their activation. This activation is important for a multitude of biological processes including regulation of glycogen sugar and lipid metabolism as well as cell cycle progression.
Pathways
The signaling related to cAMP is a major pathway where PRKAR2 is active. This protein is related to both the cAMP signaling and Ras signaling pathways. Within these networks the cAMP-mediated activation of PKA impacts the function of proteins such as CREB and MAPK influencing gene transcription and cell survival. The interaction with these pathways highlights the significant influence PRKAR2 has on cellular function and regulation.
PRKAR2 has been linked to certain health conditions like Carney complex and some types of cancer. Alterations in PRKAR2 expression or mutations can lead to dysregulation of PKA activity affecting cell proliferation and survival. The protein's interaction with other complexes such as those involving the protein PRKACA contributes to understanding these conditions. These connections suggest that targeting PRKAR2 could potentially offer therapeutic strategies for related diseases.