Abcam, ab264482, Anti-hnRNP M1-M4 antibody [HL374] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal HNRPM antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:HL374,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab264482 is the carrier-free version of
ab255958
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heterogeneous nuclear ribonucleoproteins M1-M4 often referred to as hnRNP M or hnRNPM are components of a family of RNA-binding proteins. These proteins have a molecular mass of approximately 77 kDa. They localize primarily in the nucleus but can shuttle between the nucleus and cytoplasm. hnRNP M plays a critical role in mRNA processing including splicing and transport. These proteins are ubiquitously expressed in many tissues highlighting their significance in numerous cellular processes.
Biological function summary
HnRNP M1-M4 are integral to RNA metabolism and the post-transcriptional regulation of gene expression. They participate in the spliceosome complex and influence alternative splicing events by interacting with pre-mRNA. Their binding activity modulates exon inclusion or exclusion contributing to the diversity of the proteome. hnRNP M also interacts with other RNA-binding proteins facilitating various RNA processing events.
Pathways
The activities of hnRNP M1-M4 intersect significantly with the RNA splicing machinery and mRNA transport pathways. They are integral to the spliceosome pathway and are known to interact with other splicing factors such as serine/arginine-rich proteins. This interaction is central to the regulation of alternative splicing. hnRNP M also influences the mRNA surveillance pathway ensuring proper mRNA maturation before export to the cytoplasm.
HnRNP M1-M4 are associated with cancer and neurodegenerative diseases. Aberrations in hnRNP M expression or function can lead to altered splicing patterns fostering oncogenic processes. Furthermore changes in their activity have been observed in conditions like Alzheimer's disease where they may interact with tau protein and contribute to pathogenesis by affecting tau mRNA splicing. Understanding their role in these contexts provides insights into the mechanisms underpinning these diseases and informs potential therapeutic targets.