Abcam, ab264617, Human CKM ELISA Kit

Size: 1 x 96Tests
Human CKM ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CKM with a sensitivity of 18.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 18.9 pg/mL,
Range:62.5 - 4000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CKM ELISA Kit ab264617 is a rapid single-wash 90-min Sandwich ELISA to measure Human CKM in citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 18.9 pg/mL.
How the assay works
Human CKM SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human CKM ELISA Kit ab264617 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase, is an enzyme that catalyzes the reversible transfer of a high-energy phosphate group between phosphocreatine and ATP. There are five known CK isozymes, three located in the cytosol (CKMM, CKMB, CKBB) and two in the mitochondria (MiSCK and MiuCK). CK isoenzymes allow for the generation of a large pool of phosphocreatine, considered to be a temporal and spatial ATP buffering system in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. High levels of serum CKM can be found in inherited myophathies such as Duchenne and Becker muscular dystrophy and other acquired pathologies such as rhabdomyolysis, malignant hyperthermia, inflammatory myophaties, severe trauma and acute renal failure. This kit is developed to specifically recognize only CKM.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C