Abcam, ab264754, Human POLL (DNA Polymerase lambda) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
POLL KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-POLL, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DNA polymerase lambda also known as pol λ or DNA pol λ is a DNA polymerase protein involved in DNA repair and replication processes. It has a molecular weight of approximately 65 kDa. DNA polymerase lambda is expressed in various tissues but shows a high concentration in testis and thymus indicating its role in germ cells and immune response. The enzyme exhibits both DNA polymerase and terminal transferase activities facilitating DNA synthesis across gaps and lesions.
Biological function summary
DNA polymerase lambda acts as a gap-filling enzyme during DNA repair processes especially in base excision repair and non-homologous end joining. This polymerase does not typically function within a larger complex but requires accessory factors to perform its repair functions. It maintains genomic stability by providing an accurate extension of DNA strands during repair ensuring the correct rejoining of DNA. Furthermore it also exhibits a lyase activity that can remove 5'-deoxyribose phosphate residues.
Pathways
DNA polymerase lambda is actively involved in the base excision repair and non-homologous end joining pathways. These pathways are important for fixing DNA single-strand breaks and double-strand breaks respectively. Pol λ works alongside proteins like XRCC1 in the short-patch base excision repair pathway and interacts with KU70/80 in non-homologous end joining reinforcing its multifaceted role in maintaining DNA integrity under varying genotoxic stress conditions.
DNA polymerase lambda is linked to conditions such as cancer and immunodeficiencies where its dysfunction can lead to genomic instability. The loss or aberrant function of DNA pol λ may result in ineffective DNA repair contributing to cancer development due to accumulated DNA damage. It can also interact with tumor suppressor p53 highlighting its involvement in pathways that prevent oncogenesis. Additionally impairments in its function can contribute to immunodeficiency disorders as proper DNA repair is essential for the development and function of the immune system.