Abcam, ab264758, Human MKNK1 (MNK1) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MKNK1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MKNK1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mitogen-activated protein kinase-interacting serine/threonine-protein kinase 1 (MNK1) also known as MAP kinase-interacting kinase 1 is an enzyme with a molecular weight of approximately 51 kDa. MNK1 plays an important role in the regulation of protein synthesis by phosphorylating eukaryotic initiation factor 4E (eIF4E). Researchers find high expression of MNK1 in many tissues especially within the brain and immune system which suggests its involvement in diverse cellular processes.
Biological function summary
The protein kinase MNK1 regulates the translation of mRNA by forming part of the signaling complex that includes eIF4E. This complex plays a role in stress response cell growth and the immune response. Protein synthesis regulated by MNK1 affects cellular activities by selectively translating specific mRNAs related to those processes. MNK1's specific regulation often depends on signals received through upstream kinases.
Pathways
MNK1 is a part of the mitogen-activated protein kinase (MAPK) signaling cascade particularly the extracellular signal-regulated kinase (ERK) and p38 MAPK pathways. These pathways find involvement in transmitting signals from the cell membrane to the nucleus affecting gene expression and cellular responses. MNK1 modulates its activity in response to signals from upstream proteins such as ERK1/2 or p38α/β which are likely to influence the regulation of eIF4E and therefore translation initiation.
Scientists observe MNK1 implications in cancer progression and neurodegenerative diseases. MNK1-mediated phosphorylation of proteins like eIF4E and its overactivation often correlates with tumor growth and resistance to chemotherapy. Additionally research links MNK1's altered activity to neurodegenerative conditions such as Alzheimer's disease. Interactions between MNK1 and other proteins like eIF4E suggest potential roles for MNK1 in these disease pathologies making it an attractive target for therapeutic interventions.