Abcam, ab264904, Human L3MBTL2 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
L3MBTL2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-L3MBTL2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The L3MBTL2 protein also known as Lethal(3)malignant brain tumor-like protein 2 is a chromatin-binding protein involved in transcriptional repression. It has an approximate mass of 88 kDa. L3MBTL2 plays a role in the Polycomb group of proteins which are known for chromatin modification and gene expression regulation. The protein is broadly expressed in various tissues with higher expression noted in the thymus and testis.
Biological function summary
L3MBTL2 contributes to maintaining gene silencing through its interaction with various chromatin-modifying complexes. As part of the PcG repression complex it binds to modified histones particularly those marked by methylation to suppress transcription. This role is important for proper development and cellular differentiation as it ensures genes turn off or on at the right times during cell cycle and differentiation processes.
Pathways
L3MBTL2 plays key roles in pathways controlling gene expression and cell cycle regulation. Through the Polycomb repressive complex it connects to pathways that influence cellular proliferation and differentiation. L3MBTL2 often interacts with proteins such as RING1B and EZH2 in these pathways facilitating the propagation of chromatin-associated silencing marks that help coordinate complex gene expression programs.
L3MBTL2 is linked to certain cancers and developmental disorders due to its role in gene regulation. Misregulation of L3MBTL2 has been observed in hematopoietic malignancies impacting the normal proliferation of blood cells. It shares connections with proteins like BMI1 in the context of these disorders highlighting its relevance in maintaining the balance of gene expression required for cellular homeostasis and preventing oncogenesis.