Abcam, ab265074, Human CERS6 (LASS6) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
CERS6 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-CERS6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LASS6 also known as CERS6 is a member of the ceramide synthase family which are enzymes involved in sphingolipid metabolism. CERS6 has a protein mass of approximately 44 kDa. The enzyme is predominantly expressed in the brain liver and kidneys. It operates by catalyzing the formation of ceramides with specific acyl chains playing a role in sphingolipid biosynthesis.
Biological function summary
CERS6 is involved in the synthesis of longer-chain ceramides which impacts cell membrane structure and signaling. CERS6 performs its function alone and not as part of a larger complex. The enzyme's activity influences cellular processes such as proliferation differentiation and apoptosis contributing to cellular homeostasis. Ceramides produced by CERS6 affect membrane properties and have roles in signal transduction pathways.
Pathways
CERS6 is an important player in the sphingolipid metabolism pathway affecting lipid signaling and apoptosis regulation. This enzyme collaborates with other ceramide synthases such as CERS2 in maintaining balance in ceramide levels influencing cell fate decisions. It also participates in interconnected pathways like the endoplasmic reticulum stress response interacting with proteins such as PERK to modulate apoptosis under stress conditions.
CERS6 shows a connection with cancer and neurological disorders. Its altered expression or activity links to tumorigenesis affecting cell growth and survival through interactions with proteins such as p53. In neurological diseases misregulation of CERS6 influences neurodegenerative processes potentially linking to proteins involved in neuroinflammation and cell death. Understanding CERS6's role in these diseases aids in designing therapeutic strategies targeting ceramide metabolism.