Abcam, ab265266, Human LRWD1 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
LRWD1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LRWD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The LRWD1 also known as Leucine-rich repeats and WD repeat-containing protein 1 plays an essential mechanical role in cells. This protein weighs approximately 63 kDa and is expressed mainly in tissues such as the testis. LRWD1 has structural domains that include leucine-rich repeats and WD repeat domains contributing to its role in protein interactions and signaling pathways.
Biological function summary
LRWD1 supports the regulation of DNA replication and centrosome cycle. It is an integral part of the DNA replication licensing pathway ensuring proper DNA synthesis during the cell cycle. By participating in this complex LRWD1 collaborates with proteins like CDC6 and CDT1 to initiate DNA replication. This protein ensures that cells duplicate their DNA once per cycle maintaining genomic stability.
Pathways
LRWD1 participates in the DNA replication pathway and is also linked to the G2 to M phase transition of the cell cycle. Through the DNA replication pathway LRWD1 works hand-in-hand with other factors such as ORC1 and MCM proteins which coordinate the licensing of replication origins. Its presence in these pathways demonstrates its pivotal role in maintaining normal cell cycle progression and genomic integrity.
LRWD1 has associations with disorders involving improper DNA replication and genome instability. Misregulation of this protein can relate to conditions such as cancer due to its involvement in cell cycle control. In cancer LRWD1's interaction with DNA replication-related proteins highlights the pathway's relevance in tumorigenesis. Researchers continue to investigate its role and potential connections to proteins like p53 which is frequently mutated in cancers.