Abcam, ab265279, Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SELENBP1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and 1 bp insertion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SELENBP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Selenium Binding Protein 1 (SBP1) also known as SELENBP1 is a protein of molecular weight approximately 56 kDa. It is involved in various cellular mechanisms. SBP1 binds selenium thereby playing a role in selenium metabolism and regulation within the cell. It is expressed in numerous tissues including liver kidney and colon indicating a broad physiological significance. Its expression is subject to tight regulation pointing to its importance in cellular functions.
Biological function summary
Selenium Binding Protein 1 interacts with selenium implicating it in various metabolic processes. It does not act as part of a larger complex but functions independently to influence cellular responses to selenium levels. Research shows that SBP1 plays a role in antioxidant defense and cellular detoxification suggesting a protective function against oxidative stress. This regulation supports various metabolic pathways by modulating selenium-dependent enzyme activity.
Pathways
Selenium Binding Protein 1 has been connected to the antioxidant defense pathway and the detoxification process. It interacts with proteins involved in these pathways such as glutathione peroxidases essential for eliminating oxidative damage in cells. By influencing these pathways SBP1 helps maintain cellular homeostasis highlighting its role in the broader metabolic network.
Aberrations in Selenium Binding Protein 1 levels are linked to certain cancers and neurological disorders. Studies show a decrease in SBP1 expression in cancers such as colorectal cancer possibly related to reduced cellular detoxification capacity. Additionally some neurological disorders have shown altered SBP1 expression which could connect the protein with enhanced oxidative stress and cell damage. By affecting conditions like cancer SBP1 could interact with proteins involved in cell proliferation and survival supporting research on therapeutic targets.