Abcam, ab265291, Human SCAND1 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SCAND1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SCAND1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCAND1 also known as SCAN domain-containing protein 1 has a molecular mass of approximately 20 kDa. Mechanically SCAND1 functions as a transcriptional regulator utilizing its SCAN domain to mediate protein-protein interactions and influence gene expression. Expression of SCAND1 occurs mainly in the nucleus across various cell types indicative of its role in controlling gene transcription.
Biological function summary
SCAND1 acts as a coordinator in cellular transcription machinery by interacting with other proteins. It often forms part of multiprotein complexes that govern chromatin remodeling and gene transcription. Through its interactions SCAND1 can influence the expression of genes that are critical in cell cycle regulation and development. These interactions contribute to cellular homeostasis and equilibrium.
Pathways
SCAND1 fits into gene transcription and chromatin remodeling circuits. Specifically it engages in interactions within the Notch signaling pathway and the Wnt signaling pathway. These pathways involve cooperation with other proteins like Notch receptors and beta-catenin which are pivotal in developmental processes and cell fate determination. SCAND1 modulates transcriptional responses that are delicate to environmental and intracellular cues.
SCAND1 shows a connection with cancer and neurological disorders. Alterations in SCAND1 expression or function can disturb normal transcriptional regulation contributing to tumorigenesis. In cancer SCAND1 may interact with proteins like p53 a well-known tumor suppressor protein. Misregulation of SCAND1 has been observed in some neurological disorders where its interaction with transcription factors is altered affecting neural functions. Understanding SCAND1's role in these diseases could open new avenues for therapeutic interventions.