Abcam, ab265326, Human UBE2D4 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
UBE2D4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-UBE2D4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UBE2D4 also known as UBC4/5 homolog is an enzyme with a molecular weight of approximately 16 kDa. This protein functions as a ubiquitin-conjugating enzyme that plays a role in the ubiquitination process helping to transfer ubiquitin from an E1 activating enzyme to an E3 ligase substrate protein. UBE2D4 is expressed in various tissues throughout the body including high expression in skeletal muscle and liver. Its mechanical role is critical in tagging proteins for degradation via the proteasome.
Biological function summary
The ubiquitin-conjugating enzyme plays a role in protein homeostasis by facilitating the ubiquitin-proteasome system a complex that includes E1 E2 and E3 enzymes. UBE2D4 acts in concert with specific E3 ligases to regulate cell cycle progression and transcriptional regulation. This process ensures the removal of misfolded or damaged proteins thereby maintaining protein quality within cells. Its involvement in diverse cellular pathways highlights the enzyme's importance in controlling cellular activities.
Pathways
UBE2D4 operates in the ubiquitin-mediated proteolysis and cell cycle regulatory pathways. These pathways control protein turnover and degradation ensuring cellular equilibrium. UBE2D4 collaborates with E3 ligases like MDM2 within these pathways influencing proteins like p53 which is important for cell cycle control and apoptosis. The interconnectedness with MDM2 emphasizes UBE2D4's role in key cellular events.
Alterations or malfunctions in UBE2D4 activity have linked it to cancer and neurodegenerative diseases. Misregulation of p53 related proteins via UBE2D4 alterations may promote unrestrained cell proliferation seen in cancers. Furthermore abnormal ubiquitination processes associating with pathways including UBE2D4 contribute to the accumulation of protein aggregates in neurodegenerative disorders including Alzheimer's disease. The relationship with MDM2 and p53 proteins highlights UBE2D4's significant role in disease pathogenesis.