Abcam, ab265398, Human TPGS2 (L17) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TPGS2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TPGS2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
L17 also known as ribosomal protein L17 is an important component of the 60S subunit of the eukaryotic ribosome. It has a molecular mass of approximately 22 kDa. L17 is expressed in various tissues throughout the body with particularly high expression levels in rapidly-dividing cells. As a structural element of the large ribosomal subunit L17 plays a role in the core processes of protein synthesis.
Biological function summary
The function of protein L17 involves participation in the formation of the ribosome complex. It interacts with other ribosomal proteins and rRNA molecules to ensure the accurate assembly of the ribosome. This assembly allows the ribosomal machinery to facilitate efficient translation of mRNA into polypeptides which are subsequently folded into functional proteins essential for cellular functions and proliferation.
Pathways
The protein L17 engages with translation-related pathways notably the ribosomal biogenesis pathway and the mTOR signaling pathway. The mTOR pathway regulates cell growth and metabolism in response to nutrients stress and growth factors. As part of the ribosome L17 indirectly associates with proteins like RPL5 and RPL11 essential for ribosome biogenesis and function forming a link between ribosome assembly and cellular regulation.
Ribosomal protein L17 has implications in ribosomopathies such as Diamond-Blackfan Anemia which is characterized by impaired ribosome biogenesis and function. Mutations affecting L17 and its associated proteins such as RPL5 and RPL11 can disrupt normal ribosomal biogenesis leading to developmental abnormalities and increased susceptibility to specific cancers due to the imbalance in protein synthesis and cellular homeostasis.