Abcam, ab265402, Human SPG11 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SPG11 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SPG11, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPG11 also known as spatacsin is a protein with a mass of approximately 249 kDa. This protein is expressed mainly in neural tissues including the brain and spinal cord. Mechanically SPG11 interacts with various other proteins contributing to the maintenance and function of the lysosomal compartment within cells. It plays an important role in membrane trafficking processes particularly in the vesicles involved in endocytosis and autophagy.
Biological function summary
In the context of neuronal health SPG11 is significant for the function of neurons. It forms part of a larger protein complex known as the AP-5 adaptin complex which assists in lysosomal positioning and function. By ensuring proper lysosomal function SPG11 supports neuronal maintenance and health. Issues with its function disrupt lysosomal degradation pathways and can have broad implications for neuron viability.
Pathways
SPG11 is essential in endolysosomal and autophagic pathways serving as a connection point for effective lysosomal transport. These pathways include interactions with proteins such as AP5Z1 and ZFYVE26 which collaborate to modulate lysosomal positioning and maintenance. SPG11's proper operation within these pathways allows for efficient cellular digestion of macromolecules and removal of damaged organelles critical for cellular health.
Mutations in SPG11 lead to rare neurological conditions like spastic paraplegia type 11 and some forms of juvenile amyotrophic lateral sclerosis (ALS). SPG11's mutation or dysfunction can result in improper lysosomal degradation contributing to the nervous system's degeneration. Related proteins like spastizin (ZFYVE26) and AP5Z1 when disrupted also exhibit similar symptoms of neural decay highlighting SPG11's integral role in neuronal disease mechanisms.