Abcam, ab265480, Human TRPM7 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TRPM7 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 14 and 5 bp deletion in exon 14 and 8 bp deletion in exon 14. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 14 and 5 bp deletion in exon 14 and 8 bp deletion in exon 14,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TRPM7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRPM7 also known as transient receptor potential melastatin 7 is an ion channel and a kinase with dual functionality. This protein has a mass of about 190 kDa and is widely expressed in a variety of tissues including brain heart kidney and lung. Mechanically TRPM7 serves as a channel for divalent cations primarily allowing the movement of magnesium (Mg2+) and calcium (Ca2+) ions across cellular membranes. Its kinase domain plays a role in autophosphorylation and substrate phosphorylation further influencing cellular processes.
Biological function summary
TRPM7 regulates cellular magnesium homeostasis and calcium influx in many cell types. It is integral in maintaining the cell's ion balance and influences various cellular functions like growth proliferation and apoptosis. TRPM7 is often part of larger molecular complexes that enable it to interact with other proteins and lipids modulating signal transduction pathways. It participates in mechanosensory functions as well impacting cellular responses to mechanical stimuli.
Pathways
TRPM7 is significant in the MAPK and PI3K/Akt pathways which are pivotal for cell survival and proliferation. Through these pathways TRPM7 interacts with other proteins such as TRPM6 and TRPV4. It modulates intracellular signaling cascades by regulating ion concentrations that affect protein interactions and enzyme activities thereby controlling critical cellular responses like inflammation and differentiation.
TRPM7 is linked to cancer and neurodegenerative diseases. In cancers altered TRPM7 expression promotes tumor growth and metastasis potentially interacting with proteins like Akt. In neurodegenerative conditions such as Alzheimer's disease abnormal TRPM7 function or expression contributes to neuronal death and is associated with other proteins like amyloid-beta and tau making TRPM7 a potential therapeutic target for modulation.