Abcam, ab265500, Human WRNIP1 (WHIP) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
WRNIP1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-WRNIP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The WRNIP1/WHIP protein also known as Werner helicase interacting protein 1 (WRNIP1) plays a role in DNA repair processes. It has a molecular mass of approximately 72 kDa. WRNIP1 is expressed in multiple tissues with notable expression in the nucleus of proliferating cells. Mechanically the protein interacts with the Werner syndrome ATP-dependent helicase (WRN) and modulates its activity during the processing of stalled replication forks. Its interaction with DNA binding proteins is important to maintaining genomic stability.
Biological function summary
This protein aids cellular processes involved in DNA replication and repair. WRNIP1 forms part of a complex that includes proteins involved in the stabilization and restart of collapsed replication forks. The protein facilitates the stabilization and elongation of DNA strands playing a role in homologous recombination repair. The action of WRNIP1 extends to cell cycle regulation by ensuring repair precision before mitosis proceeds.
Pathways
WRNIP1 integrates into processes linked with the replication stress response and homologous recombination repair pathways. In these pathways it collaborates with proteins such as RAD51 an essential player in homologous recombination. Additionally WRNIP1 acts in cohesion with WRN influencing pathways that mitigate the risk of cancer by preventing excessive genomic alterations. These pathways are essential for the proper resolution of replication fork stalling situations.
WRNIP1 is closely associated with Werner syndrome and cancer predisposition. Its dysfunction can lead to defects in DNA repair mechanisms increasing susceptibility to such disorders. Along with WRN any alterations could potentially lead to faulty repair of DNA damage paving a way for oncogenesis. Researchers investigate WRNIP1 concerning its protective role against replication stress-induced genomic instability and its potential implications in drug resistance within cancer treatment strategies.