Abcam, ab265501, Human C9orf69 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TMEM250 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 71 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 71 bp deletion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM250, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C9orf69 also known as Chromosome 9 Open Reading Frame 69 is a protein of approximately 38 kDa in mass. It is primarily expressed in the cytoplasm of various cell types including immune cells and certain epithelial tissues. The protein shows a ubiquitous expression pattern indicating its presence across different tissues with varying expression levels depending on the tissue type.
Biological function summary
C9orf69 plays a role in cellular processes related to immune response and cell signaling. This protein is not yet associated with any specific complex but its presence in immune cells suggests an involvement in regulation or response mechanisms. Its functions contribute to the maintenance of cellular activities especially related to homeostasis and stress responses.
Pathways
Multiple cellular processes engage C9orf69. It associates with signaling pathways that mediate cellular responses including the immune response and intracellular signaling networks. C9orf69 shows functional connectivity to proteins such as NF-kB a critical regulator of immune and inflammatory responses indicating its potential role in modulating these pathways.
Studies have linked C9orf69 to conditions such as autoimmune diseases and inflammatory disorders. Its involvement with immune cell function suggests a connection to disorders like rheumatoid arthritis and lupus where immune regulation is disrupted. Research indicates interactions with cytokine proteins such as TNF-alpha highlighting its importance in the context of inflammatory diseases.