Abcam, ab265550, Human WWC2 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
WWC2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-WWC2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WWC2 also known as WW and C2 domain-containing protein 2 is a protein with a mass of approximately 135 kDa. It functions mechanically through its WW and C2 domains that are important for protein-protein interactions. This protein is significantly expressed in various human tissues including the brain liver and heart indicating its broad role in human physiology. Through its structure WWC2 interacts with other cellular proteins potentially influencing multiple pathways.
Biological function summary
WWC2 participates in the regulation of cellular signaling and cell polarity. It is not isolated in its functioning; instead it forms complexes with other proteins such as the aPKC and LATS kinases to exert its biological effects. These interactions suggest its contribution to the Hippo signaling pathway affecting cell growth apoptosis and stem cell functions.
Pathways
WWC2 plays an important role in the Hippo pathway and possibly in cell polarity signaling. In the Hippo pathway WWC2 associates with key components such as LATS1/2 which regulates important cellular processes like proliferation and organ size. Through these interactions WWC2 influences downstream effectors such as YAP/TAZ connecting it to broader cellular outcomes. Additionally its role in cell polarity is suggested by potential interactions with aPKC indicating a role in maintaining cellular structure and function.
Mutations or dysregulation of WWC2 have associations with cancer and neurological disorders. In cancer alterations in the Hippo pathway components including WWC2 can lead to uncontrolled cell growth. While studying its role in neurological disorders scientists note its influence on neuronal cell polarity and signaling. This brings WWC2 into focus alongside proteins like YAP and TAZ which have links to various cancer types and developmental disorders.