Abcam, ab265571, Human TMEM175 knockout HeLa cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
TMEM175 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM175, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM175 also known as Transmembrane Protein 175 is a channel protein with dual function as a lysosomal potassium channel. It has an approximate mass of 66 kDa. TMEM175 plays a role in ion homeostasis and is expressed mainly in tissues with high levels of lysosomes including the brain and the immune system. This protein aids in the maintenance of the lysosomal environment important for cellular activities.
Biological function summary
This lysosomal protein contributes to the maintenance of ion stability within lysosomes an important factor in lysosomal degradation and overall cellular clearance processes. TMEM175 is an integral component of the lysosomal membrane and participates in ensuring lysosomal pH stability therefore influencing autophagic activity. TMEM175 does not participate in known protein complexes but it is pivotal for preserving lysosomal integrity.
Pathways
TMEM175 actively engages in the lysosomal degradation pathway impacting cellular waste processing. TMEM175 influences endolysosomal trafficking and autophagy which are essential for metabolite recycling. It works alongside proteins like ATPase and lysosomal enzyme hydrolases by controlling ion concentrations which can influence other lysosomal functions and pathways.
TMEM175 has a significant connection with neurodegenerative conditions such as Parkinson's disease. Mutation or dysfunction in TMEM175 impacts lysosomal function linked to protein aggregation often involving alpha-synuclein which serves as a hallmark for such neurodegenerative diseases. Additionally TMEM175 is implicated in lysosomal storage disorders where impaired lysosomal function results in cellular accumulation of substances due to ineffective degradation.